PrimePCR™ Probe Assay: C2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: C2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: C2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039848
Assay Design:   exonic
Chromosome Location:   6:31901470-31901663question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000234256 ENST00000418584 ENST00000431247 ENST00000416252 ENST00000421932 ENST00000419062 ENST00000551081 ENST00000550363 ENST00000411885 ENST00000439426 ENST00000383362 ENST00000428349 ENST00000383367 ENST00000548973 ENST00000547984 ENST00000469372 ENST00000497706 ENST00000452323 ENST00000452202 ENST00000299367 ENST00000442278 ENST00000447952 ENST00000418949 ENST00000383177 ENST00000494905 ENST00000556679 ENST00000456570 ENST00000375510 ENST00000415985 ENST00000383359 ENST00000455713 ENST

Gene Information

Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.[provided by RefSeq Mar 2009]

Gene Symbol:   C2
Gene Name:   complement component 2
Aliases:   CO2, DKFZp779M0311
RefSeq:   NC_000006.11 NG_000013.3 NG_004658.2 NG_005163.2 NG_008191.1 NG_011730.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000166278
Entrez:   717
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.030000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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