PrimePCR™ Probe Assay: CNTNAP3B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039832
Assay Design:   exonic
Chromosome Location:   9:43685170-43685267question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000394744 ENST00000444352 ENST00000400454 ENST00000568954 ENST00000393524 ENST00000567016 ENST00000356272 ENST00000275246 ENST00000359775 ENST00000380817 ENST00000348911 ENST00000390679 ENST00000377659 ENST00000377656 ENST00000354534 ENST00000545061 ENST00000377564 ENST00000276974 ENST00000432176 ENST00000440481 ENST00000409923 ENST00000409763 ENST00000377576 ENST00000570101 ENST00000219320 ENST00000496424 ENST00000318430

Gene Information

Description Not Available

Gene Symbol:   CNTNAP3B
Gene Name:   contactin associated protein-like 3B
Aliases:   DKFZp686I15204
RefSeq:   NC_000009.11 NT_167188.1
Ensembl:   ENSG00000154529
Entrez:   728577
UniGene:   Hs.521495
Chromosome Mapping:   9p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.070000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download