PrimePCR™ Probe Assay: IER3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IER3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IER3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039816
Assay Design:   exonic
Chromosome Location:   6:30711725-30711814question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000515632 ENST00000397527 ENST00000342580 ENST00000450236 ENST00000436988 ENST00000350763 ENST00000341037 ENST00000423613 ENST00000376377 ENST00000259874 ENST00000562034 ENST00000435856 ENST00000446599 ENST00000416884 ENST00000429592 ENST00000413800 ENST00000360039 ENST00000423751 ENST00000411994 ENST00000426974 ENST00000439190 ENST00000416336 ENST00000511251 ENST00000392313 ENST00000262815 ENST00000367588 ENST00000598234 ENST00000383560 ENST00000400509 ENST00000450849 ENST00000544779

Gene Information

This gene functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro but these transcripts are not found in vivo and do not generate a valid protein. [provided by RefSeq Jul 2008]

Gene Symbol:   IER3
Gene Name:   immediate early response 3
Aliases:   DIF-2, DIF2, GLY96, IEX-1, IEX-1L, IEX1, PRG1
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000137331
Entrez:   8870
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.560000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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