PrimePCR™ Probe Assay: PPAN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039808
Assay Design:   exonic
Chromosome Location:   19:10218664-10218775question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000393976 ENST00000254043 ENST00000332582 ENST00000310325 ENST00000378466 ENST00000435556 ENST00000379264 ENST00000379255 ENST00000296869 ENST00000379246 ENST00000379244 ENST00000379240 ENST00000431707 ENST00000379249 ENST00000379272 ENST00000357096 ENST00000543479 ENST00000544770 ENST00000358077 ENST00000472284 ENST00000408954 ENST00000491893 ENST00000469640 ENST00000268482 ENST00000280706 ENST00000367204 ENST00000372872 ENST00000394810 ENST00000537432 ENST00000350210 ENST00000368368 ENST

Gene Information

The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11) an adjacent downstream gene that encodes an ATP receptor has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation. [provided by RefSeq Nov 2010]

Gene Symbol:   PPAN
Gene Name:   peter pan homolog (Drosophila)
Aliases:   BXDC3, MGC14226, MGC45852, SSF, SSF-1, SSF1, SSF2
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000130810
Entrez:   56342
UniGene:   Not Available
Chromosome Mapping:   19p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 42.030000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download