PrimePCR™ Probe Assay: VCY, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: VCY, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VCY, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039804
Assay Design:   exonic
Chromosome Location:   Y:16168681-17801006question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000456197 ENST00000446306 ENST00000355540 ENST00000359863 ENST00000250825 ENST00000250823 ENST00000381032 ENST00000453306 ENST00000336812 ENST00000372873 ENST00000372872 ENST00000394810 ENST00000350061 ENST00000288139 ENST00000422281 ENST00000481478 ENST00000544977 ENST00000590341 ENST00000023064 ENST00000587772 ENST00000565218 ENST00000562259 ENST00000562468 ENST00000561982

Gene Information

The protein encoded by this gene is a member of a family of human VCX/Y genes. This gene family has multiple members on both X and Y chromosomes and all are expressed exclusively in male germ cells. Members of the VCX/Y family share a high degree of sequence identity with the exception that a 30-bp unit is tandemly repeated in X-linked members but occurs only once in Y-linked members. VCX/Y genes encode small and highly charged proteins of unknown function. This gene encodes a small positively charged protein. The presence of a putative bipartite nuclear localization signal suggests that this gene encodes a nuclear protein. The genome has two identical copies of this gene within a palindromic region; this record represents the more telomeric copy. [provided by RefSeq Jul 2008]

Gene Symbol:   VCY
Gene Name:   variable charge, Y-linked
Aliases:   BPY1, MGC119817, VCY1, VCY1A
RefSeq:   NC_000024.9 NG_004442.1 NT_011875.12
Ensembl:   ENSG00000129862
Entrez:   9084
Chromosome Mapping:   Yq11.221

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.880000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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