PrimePCR™ Probe Assay: SLC39A7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC39A7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC39A7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039792
Assay Design:   exonic
Chromosome Location:   6:33169573-33169685question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000418477 ENST00000423043 ENST00000429042 ENST00000414916 ENST00000383214 ENST00000383213 ENST00000444757 ENST00000374675 ENST00000374677 ENST00000454420 ENST00000416369 ENST00000441854 ENST00000414757 ENST00000441953 ENST00000431735 ENST00000456261 ENST00000443773 ENST00000425069

Gene Information

The protein encoded by this gene transports zinc from the Golgi and endoplasmic reticulum to the cytoplasm. This transport may be important for activation of tyrosine kinases some of which could be involved in cancer progression. Therefore modulation of the encoded protein could be useful as a therapeutic agent against cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Nov 2011]

Gene Symbol:   SLC39A7
Gene Name:   solute carrier family 39 (zinc transporter), member 7
Aliases:   D6S115E, D6S2244E, H2-KE4, HKE4, KE4, RING5, ZIP7
RefSeq:   NC_000006.11 NG_023374.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000112473
Entrez:   7922
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.940000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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