PrimePCR™ Probe Assay: TEX28P2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TEX28P2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TEX28P2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039786
Assay Design:   exonic
Chromosome Location:   X:153441181-153441312question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000445522 ENST00000448701 ENST00000443236 ENST00000354105 ENST00000371754 ENST00000371752 ENST00000371744 ENST00000455070 ENST00000396105 ENST00000586178 ENST00000415868 ENST00000376381 ENST00000399074 ENST00000457451 ENST00000399065 ENST00000573553 ENST00000571941 ENST00000377424 ENST00000330154 ENST00000397977 ENST00000399409 ENST00000216840 ENST00000560777 ENST00000367187 ENST00000424712 ENST00000411959 ENST00000369941 ENST00000369932 ENST00000369926 ENST00000361930 ENST00000244458 ENST

Gene Information

Description Not Available

Gene Symbol:   TEX28P2
Gene Name:   testis expressed 28 pseudogene 2
Aliases:   CXorf2B, pTEX
RefSeq:   NC_000023.10 NG_005788.1 NG_009105.1 NG_011606.1 NT_167198.1
Ensembl:   ENSG00000102080
Entrez:   653363
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.530000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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