PrimePCR™ Probe Assay: GGTLC2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GGTLC2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GGTLC2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039784
Assay Design:   exonic
Chromosome Location:   22:22988852-22988941question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000248923 ENST00000412658 ENST00000400382 ENST00000400380 ENST00000401885 ENST00000404532 ENST00000403838 ENST00000404223 ENST00000404920 ENST00000400383 ENST00000406383 ENST00000404912 ENST00000424787 ENST00000405188 ENST00000401924 ENST00000424627 ENST00000286890 ENST00000278765 ENST00000335694 ENST00000480559 ENST00000448514

Gene Information

Gamma-glutamyltransferase-1 (GGT1; MIM 612346) is a membrane-bound extracellular enzyme that cleaves gamma-glutamyl peptide bonds in glutathione and other peptides and transfers the gamma-glutamyl moiety to acceptors. Autocatalytic cleavage of the GGT1 precursor polypeptide produces a heavy chain and a light chain that associate with each other to form the functional enzyme. Light chain-only GGTs such as GGTLC2 contain a region corresponding to the GGT1 light chain but they lack the membrane-anchoring heavy chain region (Heisterkamp et al. 2008 [PubMed 18357469]).[supplied by OMIM Oct 2008]

Gene Symbol:   GGTLC2
Gene Name:   gamma-glutamyltransferase light chain 2
Aliases:   GGTL4, MGC96916
RefSeq:   NC_000022.10 NG_000002.1 NT_011520.12
Ensembl:   ENSG00000100121
Entrez:   91227
Chromosome Mapping:   22q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.900000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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