PrimePCR™ Probe Assay: CNOT7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039686
Assay Design:   exonic
Chromosome Location:   8:17102598-17102711question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000361272 ENST00000523917 ENST00000524358 ENST00000520178

Gene Information

The protein encoded by this gene binds to an anti-proliferative protein B-cell translocation protein 1 which negatively regulates cell proliferation. Binding of the two proteins which is driven by phosphorylation of the anti-proliferative protein causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   CNOT7
Gene Name:   CCR4-NOT transcription complex, subunit 7
Aliases:   CAF1, hCAF-1
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000198791
Entrez:   29883
UniGene:   Hs.645009
Chromosome Mapping:   8p22-p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.950000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download