PrimePCR™ Probe Assay: SLC9A8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SLC9A8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC9A8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039675
Assay Design:   exonic
Chromosome Location:   20:48504901-48505038question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000361573 ENST00000417961 ENST00000539601 ENST00000541138

Gene Information

Sodium-hydrogen exchangers (NHEs) such as SLC9A8 are integral transmembrane proteins that exchange extracellular Na+ for intracellular H+. NHEs have multiple functions including intracellular pH homeostasis cell volume regulation and electroneutral NaCl absorption in epithelia (Xu et al. 2008 [PubMed 18209477]).[supplied by OMIM Apr 2009]

Gene Symbol:   SLC9A8
Gene Name:   solute carrier family 9 (sodium/hydrogen exchanger), member 8
Aliases:   DKFZp686C03237, FLJ42500, KIAA0939, MGC138418, NHE8
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000197818
Entrez:   23315
Chromosome Mapping:   20q13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.160000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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