PrimePCR™ Probe Assay: ATP6V0E2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039510
Assay Design:   exonic
Chromosome Location:   7:149576937-149577040question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000421974 ENST00000456496 ENST00000479613 ENST00000425642

Gene Information

Multisubunit vacuolar-type proton pumps or H(+)-ATPases acidify various intracellular compartments such as vacuoles clathrin-coated and synaptic vesicles endosomes lysosomes and chromaffin granules. H(+)-ATPases are also found in plasma membranes of specialized cells where they play roles in urinary acidification bone resorption and sperm maturation. Multiple subunits form H(+)-ATPases with proteins of the V1 class hydrolyzing ATP for energy to transport H+ and proteins of the V0 class forming an integral membrane domain through which H+ is transported. ATP6V0E2 encodes an isoform of the H(+)-ATPase V0 e subunit an essential proton pump component (Blake-Palmer et al. 2007 [PubMed 17350184]).[supplied by OMIM Mar 2008]

Gene Symbol:   ATP6V0E2
Gene Name:   ATPase, H+ transporting V0 subunit e2
Aliases:   ATP6V0E2L, C7orf32
RefSeq:   NC_000007.13 NT_007914.15
Ensembl:   ENSG00000171130
Entrez:   155066
UniGene:   Hs.698060
Chromosome Mapping:   7q36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.870000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download