This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: ATP6V0E2, Human
PrimePCR™ Template for Probe Assay: ATP6V0E2, Human
Multisubunit vacuolar-type proton pumps or H(+)-ATPases acidify various intracellular compartments such as vacuoles clathrin-coated and synaptic vesicles endosomes lysosomes and chromaffin granules. H(+)-ATPases are also found in plasma membranes of specialized cells where they play roles in urinary acidification bone resorption and sperm maturation. Multiple subunits form H(+)-ATPases with proteins of the V1 class hydrolyzing ATP for energy to transport H+ and proteins of the V0 class forming an integral membrane domain through which H+ is transported. ATP6V0E2 encodes an isoform of the H(+)-ATPase V0 e subunit an essential proton pump component (Blake-Palmer et al. 2007 [PubMed 17350184]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.