PrimePCR™ Probe Assay: TRIM58, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039420
Assay Design:   exonic
Chromosome Location:   1:248040885-248040996question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000366481

Gene Information

Description Not Available

Gene Symbol:   TRIM58
Gene Name:   tripartite motif containing 58
Aliases:   BIA2, DKFZp434C091, DKFZp686N09198
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000162722
Entrez:   25893
UniGene:   Not Available
Chromosome Mapping:   1q44

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.920000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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