This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: SIGMAR1, Human
PrimePCR™ Template for Probe Assay: SIGMAR1, Human
This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine immune and nervous systems. As indicated by its previous name opioid receptor sigma 1 (OPRS1) the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Alternative splicing of this gene results in transcript variants encoding distinct isoforms. [provided by RefSeq Dec 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.