PrimePCR™ Probe Assay: NOVA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NOVA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NOVA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039310
Assay Design:   exonic
Chromosome Location:   14:26949203-27064617question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000465357 ENST00000539517 ENST00000449198 ENST00000347476 ENST00000549146 ENST00000549571 ENST00000344429 ENST00000547619 ENST00000574031 ENST00000267422

Gene Information

This gene encodes a neuron-specific RNA-binding protein a member of the Nova family of paraneoplastic disease antigens that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia breast cancer and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   NOVA1
Gene Name:   neuro-oncological ventral antigen 1
Aliases:   Nova-1
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000139910
Entrez:   4857
Chromosome Mapping:   14q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.470000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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