PrimePCR™ Probe Assay: ST3GAL3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ST3GAL3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ST3GAL3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039226
Assay Design:   exonic
Chromosome Location:   1:44201907-44201999question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000361392 ENST00000361400 ENST00000347631 ENST00000361812 ENST00000372362 ENST00000531451 ENST00000372369 ENST00000361746 ENST00000372367 ENST00000372366 ENST00000372365 ENST00000372368 ENST00000372372 ENST00000528371 ENST00000531993 ENST00000533933 ENST00000332628 ENST00000531816 ENST00000262915 ENST00000372375 ENST00000351035 ENST00000372374 ENST00000353126 ENST00000545417 ENST00000330208 ENST00000335430 ENST00000372377

Gene Information

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi apparatus but can be proteolytically processed to a soluble form. This protein is a member of glycosyltransferase family 29. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ST3GAL3
Gene Name:   ST3 beta-galactoside alpha-2,3-sialyltransferase 3
Aliases:   SIAT6, ST3GALII, ST3GalIII, ST3N
RefSeq:   NC_000001.10 NT_032977.9 NG_028196.1
Ensembl:   ENSG00000126091
Entrez:   6487
Chromosome Mapping:   1p34.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.400000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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