PrimePCR™ Probe Assay: PLEKHB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PLEKHB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PLEKHB2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039161
Assay Design:   exonic
Chromosome Location:   2:131883359-131883469question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000409158 ENST00000234115 ENST00000404460 ENST00000409612 ENST00000409279 ENST00000303908 ENST00000403716 ENST00000439822 ENST00000438882 ENST00000538982

Gene Information

Description Not Available

Gene Symbol:   PLEKHB2
Gene Name:   pleckstrin homology domain containing, family B (evectins) member 2
Aliases:   EVT2, FLJ20783, FLJ23679, FLJ30436
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000115762
Entrez:   55041
Chromosome Mapping:   2q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.280000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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