PrimePCR™ Probe Assay: RLN1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RLN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RLN1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039125
Assay Design:   exonic
Chromosome Location:   9:5335233-5335351question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000223862 ENST00000223858

Gene Information

Relaxins are known endocrine and autocrine/paracrine hormones belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes RLN1 RLN2 and RLN3. RLN1 and RLN2 share high sequence homology. This encoded protein is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds; however their exact cleavage sites have not been described. Relaxin is produced by the ovary and targets the mammalian reproductive system to ripen the cervix elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility regulating blood pressure controlling heart rate and releasing oxytocin and vasopressin. This gene has multiple polyadenylation sites. There are multiple alternatively spliced transcript variants described for this gene but their full length nature is not known yet. [provided by RefSeq Jul 2008]

Gene Symbol:   RLN1
Gene Name:   relaxin 1
Aliases:   H1, RLXH1, bA12D24.3.1, bA12D24.3.2
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000107018
Entrez:   6013
Chromosome Mapping:   9p24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.690000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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