This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: RLN1, Human
PrimePCR™ Template for Probe Assay: RLN1, Human
Relaxins are known endocrine and autocrine/paracrine hormones belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes RLN1 RLN2 and RLN3. RLN1 and RLN2 share high sequence homology. This encoded protein is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds; however their exact cleavage sites have not been described. Relaxin is produced by the ovary and targets the mammalian reproductive system to ripen the cervix elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility regulating blood pressure controlling heart rate and releasing oxytocin and vasopressin. This gene has multiple polyadenylation sites. There are multiple alternatively spliced transcript variants described for this gene but their full length nature is not known yet. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.