This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: WFDC2, Human
PrimePCR™ Template for Probe Assay: WFDC2, Human
This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain or WAP Signature motif contains eight cysteines forming four disulfide bonds at the core of the protein and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein which may be involved in sperm maturation. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.