PrimePCR™ Probe Assay: ALS2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ALS2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ALS2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039009
Assay Design:   exonic
Chromosome Location:   2:202626474-202631979question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000264276 ENST00000467448 ENST00000409632 ENST00000410052

Gene Information

The protein encoded by this gene contains an ATS1/RCC1-like domain a RhoGEF domain and a vacuolar protein sorting 9 (VPS9) domain all of which are guanine-nucleotide exchange factors that activate members of the Ras superfamily of GTPases. The protein functions as a guanine nucleotide exchange factor for the small GTPase RAB5. The protein localizes with RAB5 on early endosomal compartments and functions as a modulator for endosomal dynamics. Mutations in this gene result in several forms of juvenile lateral sclerosis and infantile-onset ascending spastic paralysis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2008]

Gene Symbol:   ALS2
Gene Name:   amyotrophic lateral sclerosis 2 (juvenile)
Aliases:   ALS2CR6, ALSJ, FLJ31851, IAHSP, KIAA1563, MGC87187, PLSJ
RefSeq:   NC_000002.11 NT_005403.17 NG_008775.1 NG_012654.1
Ensembl:   ENSG00000003393
Entrez:   57679
Chromosome Mapping:   2q33.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.630000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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