PrimePCR™ Probe Assay: SOSTDC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0035550
Assay Design:   Exonic
Chromosome Location:   7:16505270-16505376question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000307068 ENST00000396652

Gene Information

This gene is a member of the sclerostin family and encodes an N-glycosylated secreted protein with a C-terminal cystine knot-like domain. This protein functions as a bone morphogenetic protein (BMP) antagonist. Specifically it directly associates with BMPs prohibiting them from binding their receptors thereby regulating BMP signaling during cellular proliferation differentiation and programmed cell death. [provided by RefSeq Jul 2008]

Gene Symbol:   SOSTDC1
Gene Name:   sclerostin domain containing 1
Aliases:   DKFZp564D206, ECTODIN, USAG1
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000171243
Entrez:   25928
UniGene:   Hs.648106
Chromosome Mapping:   7p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.080000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download