PrimePCR™ Probe Assay: C14orf159, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: C14orf159, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: C14orf159, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0033385
Assay Design:   Exonic
Chromosome Location:   14:91655355-91662737question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000520328 ENST00000256324 ENST00000521077 ENST00000518868 ENST00000523816 ENST00000517518 ENST00000428926 ENST00000522322 ENST00000523771 ENST00000412671 ENST00000525393

Gene Information

Description Not Available

Gene Symbol:   C14orf159
Gene Name:   chromosome 14 open reading frame 159
Aliases:   C14orf160, DKFZp686I02128, FLJ20950, FLJ39943, FLJ39975
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000133943
Entrez:   80017
Chromosome Mapping:   14q32.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.650000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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