PrimePCR™ Probe Assay: AC006273.1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032974
Assay Design:   Exonic
Chromosome Location:   19:815685-815845question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000300947 ENST00000521445 ENST00000359894 ENST00000520876

Gene Information

The proteins in the lipid phosphate phosphatase (LPP) family including PRG2 are integral membrane proteins that modulate bioactive lipid phosphates including phosphatidate lysophosphatidate and sphingosine-1-phosphate in the context of cell migration neurite retraction and mitogenesis (Brauer et al. 2003 [PubMed 12730698]).[supplied by OMIM Mar 2008]

Gene Symbol:   AC006273.1
Gene Name:   Lipid phosphate phosphatase-related protein type 3
Aliases:   LPR3, PRG-2, PRG2
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000129951
Entrez:   79948
UniGene:   Hs.734643
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.260000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download