PrimePCR™ Probe Assay: NEU4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NEU4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NEU4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032802
Assay Design:   Exonic
Chromosome Location:   2:242757433-242757528question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000407683 ENST00000415936 ENST00000405370 ENST00000404257 ENST00000391969 ENST00000325935 ENST00000426032 ENST00000420288 ENST00000472793

Gene Information

The protein encoded by this gene belongs to a family of glycohydrolytic enzymes which remove terminal sialic acid residues from various sialo derivatives such as glycoproteins glycolipids oligosaccharides and gangliosides. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Nov 2009]

Gene Symbol:   NEU4
Gene Name:   sialidase 4
Aliases:   FLJ35928, MGC102757, MGC18222
RefSeq:   NC_000002.11 NT_005416.13
Ensembl:   ENSG00000204099
Entrez:   129807
Chromosome Mapping:   2q37.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.030000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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