PrimePCR™ Probe Assay: DMGDH, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DMGDH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DMGDH, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032595
Assay Design:   Exonic
Chromosome Location:   5:78324256-78324446question
Amplicon Length:   161
Splice Variants Targeted:   ENST00000255189 ENST00000523732 ENST00000380311 ENST00000540686 ENST00000539598

Gene Information

This gene encodes an enzyme involved in the catabolism of choline catalyzing the oxidative demethylation of dimethylglycine to form sarcosine. The enzyme is found as a monomer in the mitochondrial matrix and uses flavin adenine dinucleotide and folate as cofactors. Mutation in this gene causes dimethylglycine dehydrogenase deficiency characterized by a fishlike body odor chronic muscle fatigue and elevated levels of the muscle form of creatine kinase in serum. [provided by RefSeq Jul 2008]

Gene Symbol:   DMGDH
Gene Name:   dimethylglycine dehydrogenase
Aliases:   DMGDHD, ME2GLYDH
RefSeq:   NC_000005.9 NG_012164.1 NT_006713.15
Ensembl:   ENSG00000132837
Entrez:   29958
Chromosome Mapping:   5q14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.190000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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