PrimePCR™ Probe Assay: PSENEN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032553
Assay Design:   Exonic
Chromosome Location:   19:36237336-36237713question
Amplicon Length:   164
Splice Variants Targeted:   ENST00000222266

Gene Information

Presenilins which are components of the gamma-secretase protein complex are required for intramembranous processing of some type I transmembrane proteins such as the Notch proteins and the beta-amyloid precursor protein. Signaling by Notch receptors mediates a wide range of developmental cell fates. Processing of the beta-amyloid precursor protein generates neurotoxic amyloid beta peptides the major component of senile plaques associated with Alzheimer's disease. This gene encodes a protein that is required for Notch pathway signaling and for the activity and accumulation of gamma-secretase. [provided by RefSeq Jul 2008]

Gene Symbol:   PSENEN
Gene Name:   presenilin enhancer 2 homolog (C. elegans)
Aliases:   MSTP064, PEN-2, PEN2
RefSeq:   NC_000019.9 NG_027934.1 NT_011109.16
Ensembl:   ENSG00000205155
Entrez:   55851
UniGene:   Hs.534465
Chromosome Mapping:   19q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.800000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download