This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: PGK2, Human
PrimePCR™ Template for Probe Assay: PGK2, Human
This gene is intronless arose via retrotransposition of the phosphoglycerate kinase 1 gene and is expressed specifically in the testis. Initially assumed to be a pseudogene the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 13-bisphosphoglycerate to 3-phosphoglycerate during the Embden-Meyerhof-Parnas pathway of glycolysis in the later stages of spermatogenesis.[provided by RefSeq May 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.