This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: CITED4, Human
PrimePCR™ Template for Probe Assay: CITED4, Human
The protein encoded by this intronless gene belongs to the CITED family of transcriptional coactivators that bind to several proteins including CREB-binding protein (CBP) and p300 via a conserved 32 aa C-terminal motif and regulate gene transcription. This protein also interacts with transcription factor AP2 (TFAP2) and thus may function as a co-activator for TFAP2. Hypermethylation and transcriptional downregulation of this gene has been observed in oligodendroglial tumors with deletions of chromosomal arms 1p and 19q and associated with longer recurrence-free and overall survival of patients with oligodendroglial tumors. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.