PrimePCR™ Probe Assay: ADRB2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032222
Assay Design:   Exonic
Chromosome Location:   5:148206939-148207050question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000305988

Gene Information

This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein an adenylyl cyclase cAMP-dependent kinase and the counterbalancing phosphatase PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms point mutations and/or downregulation of this gene are associated with nocturnal asthma obesity and type 2 diabetes. [provided by RefSeq Jul 2008]

Gene Symbol:   ADRB2
Gene Name:   adrenergic, beta-2-, receptor, surface
Aliases:   ADRB2R, ADRBR, B2AR, BAR, BETA2AR
RefSeq:   NC_000005.9 NG_016421.1 NT_029289.11
Ensembl:   ENSG00000169252
Entrez:   154
UniGene:   Hs.2551
Chromosome Mapping:   5q31-q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.510000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download