This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: PSMB11, Human
PrimePCR™ Template for Probe Assay: PSMB11, Human
Proteasomes generate peptides that are presented by major histocompatibility complex (MHC) I molecules to other cells of the immune system. Proteolysis is conducted by 20S proteasomes complexes of 28 subunits arranged as a cylinder in 4 heteroheptameric rings: alpha-1 to -7 beta-1 to -7 beta-1 to -7 and alpha-1 to -7. The catalytic subunits are beta-1 (PSMB6; MIM 600307) beta-2 (PSMB7; MIM 604030) and beta-5 (PSMB5; MIM 600306). Three additional subunits beta-1i (PSMB9; MIM 177045) beta-2i (PSMB10; MIM 176847) and beta-5i (PSMB8; MIM 177046) are induced by gamma-interferon (IFNG; MIM 147570) and are preferentially incorporated into proteasomes to make immunoproteasomes. PSMB11 or beta-5t is a catalytic subunit expressed exclusively in cortical thymic epithelial cells (Murata et al. 2007 [PubMed 17540904]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.