PrimePCR™ Probe Assay: TACSTD2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032007
Assay Design:   Exonic
Chromosome Location:   1:59041385-59041523question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000371225

Gene Information

This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.[provided by RefSeq Dec 2009]

Gene Symbol:   TACSTD2
Gene Name:   tumor-associated calcium signal transducer 2
Aliases:   EGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TROP2
RefSeq:   NC_000001.10 NG_016237.1 NT_032977.9
Ensembl:   ENSG00000184292
Entrez:   4070
UniGene:   Hs.23582
Chromosome Mapping:   1p32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.120000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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