This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: IGFALS, Human
PrimePCR™ Template for Probe Assay: IGFALS, Human
The protein encoded by this gene is a serum protein that binds insulin-like growth factors increasing their half-life and their vascular localization. Production of the encoded protein which contains twenty leucine-rich repeats is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency which maifests itself in a delayed and slow puberty. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Mar 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.