PrimePCR™ Probe Assay: C7orf31, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025848
Assay Design:   Exonic
Chromosome Location:   7:25218829-25219699question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000409280 ENST00000283905 ENST00000443822 ENST00000415598 ENST00000444434

Gene Information

Description Not Available

Gene Symbol:   C7orf31
Gene Name:   chromosome 7 open reading frame 31
Aliases:   Not Available
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000153790
Entrez:   136895
UniGene:   Hs.122055
Chromosome Mapping:   7p15.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.660000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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