PrimePCR™ Probe Assay: CSRNP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025846
Assay Design:   Exonic
Chromosome Location:   12:51470194-51470283question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000228515 ENST00000548981 ENST00000552899 ENST00000546935

Gene Information

The protein encoded by this gene belongs to the CSRNP family of nuclear proteins that share conserved regions including cysteine- and serine- rich regions a basic domain a transcriptional activation domain and bind the sequence 'AGAGTG' thus have the hallmark of transcription factors. Studies in mice suggest that these genes may have redundant functions. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   CSRNP2
Gene Name:   cysteine-serine-rich nuclear protein 2
Aliases:   C12orf2, C12orf22, FAM130A1, FLJ25576, TAIP-12
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000110925
Entrez:   81566
UniGene:   Hs.524425
Chromosome Mapping:   12q13.11-q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.670000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download