PrimePCR™ Probe Assay: ARAP3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025813
Assay Design:   Exonic
Chromosome Location:   5:141053221-141053346question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000522690 ENST00000508305 ENST00000239440 ENST00000504448

Gene Information

This gene encodes a phosphoinositide binding protein containing ARF-GAP RHO-GAP RAS-associating and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(345)P3/PtdIns(34)P2-stimulated Arf6-GAP protein. An alternatively spliced transcript has been found for this gene but its biological validity has not been determined. [provided by RefSeq Sep 2008]

Gene Symbol:   ARAP3
Gene Name:   ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
Aliases:   CENTD3, DRAG1, FLJ21065
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000120318
Entrez:   64411
UniGene:   Hs.726187
Chromosome Mapping:   5q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.310000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download