PrimePCR™ Probe Assay: ARFGAP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025795
Assay Design:   Exonic
Chromosome Location:   11:47198106-47198344question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000524782 ENST00000526342 ENST00000527927 ENST00000525398 ENST00000525314 ENST00000528444 ENST00000530596 ENST00000426335 ENST00000419701 ENST00000319543

Gene Information

Description Not Available

Gene Symbol:   ARFGAP2
Gene Name:   ADP-ribosylation factor GTPase activating protein 2
Aliases:   FLJ14576, FLJ26000, IRZ, ZNF289, Zfp289
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000149182
Entrez:   84364
UniGene:   Hs.436204
Chromosome Mapping:   11p11.2-p11.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.160000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download