PrimePCR™ Probe Assay: MLF2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025793
Assay Design:   Exonic
Chromosome Location:   12:6859381-6859881question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000203630 ENST00000542154 ENST00000539187 ENST00000537126 ENST00000540710 ENST00000435120

Gene Information

Description Not Available

Gene Symbol:   MLF2
Gene Name:   myeloid leukemia factor 2
Aliases:   NTN4
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000089693
Entrez:   8079
Chromosome Mapping:   12p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.770000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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