PrimePCR™ Probe Assay: CCT3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025786
Assay Design:   Exonic
Chromosome Location:   1:156294763-156303340question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000295688 ENST00000368259 ENST00000472765 ENST00000413555 ENST00000496684 ENST00000533194 ENST00000446905 ENST00000478640 ENST00000415548 ENST00000368261

Gene Information

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT) also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition a pseudogene of this gene has been found on chromosome 8. [provided by RefSeq Aug 2010]

Gene Symbol:   CCT3
Gene Name:   chaperonin containing TCP1, subunit 3 (gamma)
Aliases:   CCT-gamma, CCTG, PIG48, TCP-1-gamma, TRIC5
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000163468
Entrez:   7203
UniGene:   Hs.491494
Chromosome Mapping:   1q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.910000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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