PrimePCR™ Probe Assay: GAS2L1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GAS2L1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GAS2L1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025707
Assay Design:   Exonic
Chromosome Location:   22:29708507-29708663question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000407647 ENST00000406549 ENST00000403764 ENST00000471961 ENST00000407854 ENST00000333679 ENST00000360113

Gene Information

The protein encoded by this gene a member of the GAS2 family is similar in sequence to the mouse protein Gas2 an actin-associated protein expressed at high levels in growth-arrested cells. Expression of the mouse Gas2 gene is negatively regulated by serum and growth factors. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   GAS2L1
Gene Name:   growth arrest-specific 2 like 1
Aliases:   GAR22, MGC17243
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000185340
Entrez:   10634
Chromosome Mapping:   22q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.310000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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