PrimePCR™ Probe Assay: ADD2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ADD2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ADD2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025665
Assay Design:   Exonic
Chromosome Location:   2:70933376-70933501question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000264439 ENST00000356565 ENST00000517596 ENST00000425976 ENST00000415348 ENST00000430656 ENST00000413157 ENST00000264436 ENST00000407644 ENST00000456320 ENST00000355733 ENST00000522886

Gene Information

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin originally purified from human erythrocytes was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C the binding site for calmodulin and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq Jun 2010]

Gene Symbol:   ADD2
Gene Name:   adducin 2 (beta)
Aliases:   ADDB
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000075340
Entrez:   119
Chromosome Mapping:   2p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.810000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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