This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: TNNC1, Human
PrimePCR™ Template for Probe Assay: TNNC1, Human
Troponin is a central regulatory protein of striated muscle contraction and together with tropomyosin is located on the actin filament. Troponin consists of 3 subunits: TnI which is the inhibitor of actomyosin ATPase; TnT which contains the binding site for tropomyosin; and TnC the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI thus allowing the interaction of actin with myosin the hydrolysis of ATP and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq Oct 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.