PrimePCR™ Probe Assay: TNNC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TNNC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TNNC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025562
Assay Design:   Exonic
Chromosome Location:   3:52485765-52485860question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000232975 ENST00000496590

Gene Information

Troponin is a central regulatory protein of striated muscle contraction and together with tropomyosin is located on the actin filament. Troponin consists of 3 subunits: TnI which is the inhibitor of actomyosin ATPase; TnT which contains the binding site for tropomyosin; and TnC the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI thus allowing the interaction of actin with myosin the hydrolysis of ATP and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq Oct 2008]

Gene Symbol:   TNNC1
Gene Name:   troponin C type 1 (slow)
Aliases:   CMD1Z, CMH13, TN-C, TNC, TNNC
RefSeq:   NC_000003.11 NG_008963.1 NT_022517.18
Ensembl:   ENSG00000114854
Entrez:   7134
Chromosome Mapping:   3p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.380000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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