This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: COL9A2, Human
PrimePCR™ Template for Probe Assay: COL9A2, Human
This gene encodes one of the three alpha chains of type IX collagen the major collagen component of hyaline cartilage. Type IX collagen a heterotrimeric molecule is usually found in tissues containing type II collagen a fibrillar collagen. This chain is unusual in that unlike the other two type IX alpha chains it contains a covalently attached glycosaminoglycan side chain. Mutations in this gene are associated with multiple epiphyseal dysplasia. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.