PrimePCR™ Probe Assay: MED13L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025246
Assay Design:   Exonic
Chromosome Location:   12:116420215-116420381question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000281928 ENST00000552340 ENST00000549786

Gene Information

The protein encoded by this gene is a subunit of the Mediator complex a large complex of proteins that functions as a transcriptional coactivator for most RNA polymerase II-transcribed genes. The encoded protein is involved in early development of the heart and brain. Defects in this gene are a cause of transposition of the great arteries dextro-looped (DTGA).[provided by RefSeq Jul 2010]

Gene Symbol:   MED13L
Gene Name:   mediator complex subunit 13-like
Aliases:   DKFZp781D0112, FLJ21627, KIAA1025, PROSIT240, THRAP2, TRAP240L
RefSeq:   NC_000012.11 NG_023366.1 NT_009775.17
Ensembl:   ENSG00000123066
Entrez:   23389
UniGene:   Hs.603766
Chromosome Mapping:   12q24.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 34.360000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download