PrimePCR™ Probe Assay: SUN2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SUN2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SUN2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025190
Assay Design:   Exonic
Chromosome Location:   22:39146233-39146915question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000405510 ENST00000405018 ENST00000406622 ENST00000430185 ENST00000438058 ENST00000456894 ENST00000420859 ENST00000411587 ENST00000216064

Gene Information

SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope known as the LINC complex via interaction with the conserved luminal KASH domain of nesprins (e.g. SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton which contributes to nuclear positioning and cellular rigidity (summary by Haque et al. 2010 [PubMed 19933576]).[supplied by OMIM Nov 2010]

Gene Symbol:   SUN2
Gene Name:   Sad1 and UNC84 domain containing 2
Aliases:   KIAA0668, MGC133055, MGC133056, UNC84B
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100242
Entrez:   25777
Chromosome Mapping:   22q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.270000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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