PrimePCR™ Probe Assay: CCT5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CCT5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CCT5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025181
Assay Design:   Exonic
Chromosome Location:   5:10264768-10264959question
Amplicon Length:   162
Splice Variants Targeted:   ENST00000280326 ENST00000503026 ENST00000515390 ENST00000515676 ENST00000506600 ENST00000440011

Gene Information

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT) also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. In addition three pseudogenes of this gene have been identified. [provided by RefSeq Jun 2010]

Gene Symbol:   CCT5
Gene Name:   chaperonin containing TCP1, subunit 5 (epsilon)
Aliases:   CCT-epsilon, CCTE, KIAA0098, TCP-1-epsilon
RefSeq:   NC_000005.9 NG_012160.1 NT_006576.16
Ensembl:   ENSG00000150753
Entrez:   22948
Chromosome Mapping:   5p15.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.710000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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