This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: TPCN1, Human
PrimePCR™ Template for Probe Assay: TPCN1, Human
Voltage-gated Ca(2+) and Na+ channels have 4 homologous domains each containing 6 transmembrane segments S1 to S6. TPCN1 is similar to these channels but it has only 2 domains containing S1 to S6 (Ishibashi et al. 2000 [PubMed 10753632]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.