PrimePCR™ Probe Assay: STIM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: STIM1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: STIM1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025105
Assay Design:   Exonic
Chromosome Location:   11:3988807-4045106question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000525403 ENST00000300737 ENST00000527651 ENST00000532610 ENST00000532919 ENST00000530554 ENST00000524822 ENST00000525055 ENST00000528656 ENST00000532990

Gene Information

This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5 an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome Wilms tumor rhabdomyosarcoma adrenocrotical carcinoma and lung ovarian and breast cancer. This gene may play a role in malignancies and disease that involve this region as well as early hematopoiesis by mediating attachment to stromal cells. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1) with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene.[provided by RefSeq Jan 2009]

Gene Symbol:   STIM1
Gene Name:   stromal interaction molecule 1
Aliases:   D11S4896E, GOK
RefSeq:   NC_000011.9 NG_016277.1 NG_027992.1 NT_009237.18
Ensembl:   ENSG00000167323
Entrez:   6786
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.690000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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