PrimePCR™ Probe Assay: XYLT2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: XYLT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: XYLT2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025093
Assay Design:   Exonic
Chromosome Location:   17:48433219-48433449question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000017003 ENST00000507602

Gene Information

The protein encoded by this gene is an isoform of xylosyltransferase which belongs to a family of glycosyltransferases. This enzyme transfers xylose from UDP-xylose to specific serine residues of the core protein and initiates the biosynthesis of glycosaminoglycan chains in proteoglycans including chondroitin sulfate heparan sulfate heparin and dermatan sulfate. The enzyme activity which is increased in scleroderma patients is a diagnostic marker for the determination of sclerotic activity in systemic sclerosis. [provided by RefSeq Jul 2008]

Gene Symbol:   XYLT2
Gene Name:   xylosyltransferase II
Aliases:   PXYLT2, XT-II, XT2, xylT-II
RefSeq:   NC_000017.10 NG_012175.1 NT_010783.15
Ensembl:   ENSG00000015532
Entrez:   64132
Chromosome Mapping:   17q21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.030000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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