PrimePCR™ Probe Assay: STAB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: STAB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: STAB1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025089
Assay Design:   Exonic
Chromosome Location:   3:52556838-52557042question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000321725

Gene Information

This gene encodes a large transmembrane receptor protein which may function in angiogenesis lymphocyte homing cell adhesion or receptor scavenging. The protein contains 7 fasciclin 16 epidermal growth factor (EGF)-like and 2 laminin-type EGF-like domains as well as a C-type lectin-like hyaluronan-binding Link module. The protein is primarily expressed on sinusoidal endothelial cells of liver spleen and lymph node. The receptor has been shown to endocytose ligands such as low density lipoprotein Gram-positive and Gram-negative bacteria and advanced glycosylation end products. Supporting its possible role as a scavenger receptor the protein rapidly cycles between the plasma membrane and early endosomes. [provided by RefSeq Jul 2008]

Gene Symbol:   STAB1
Gene Name:   stabilin 1
Aliases:   CLEVER-1, FEEL-1, FELE-1, FEX1, KIAA0246, STAB-1
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000010327
Entrez:   23166
Chromosome Mapping:   3p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.930000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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