This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: STAB1, Human
PrimePCR™ Template for Probe Assay: STAB1, Human
This gene encodes a large transmembrane receptor protein which may function in angiogenesis lymphocyte homing cell adhesion or receptor scavenging. The protein contains 7 fasciclin 16 epidermal growth factor (EGF)-like and 2 laminin-type EGF-like domains as well as a C-type lectin-like hyaluronan-binding Link module. The protein is primarily expressed on sinusoidal endothelial cells of liver spleen and lymph node. The receptor has been shown to endocytose ligands such as low density lipoprotein Gram-positive and Gram-negative bacteria and advanced glycosylation end products. Supporting its possible role as a scavenger receptor the protein rapidly cycles between the plasma membrane and early endosomes. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.