PrimePCR™ Probe Assay: SHQ1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SHQ1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SHQ1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0025054
Assay Design:   Exonic
Chromosome Location:   3:72842071-72861831question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000325599 ENST00000463369

Gene Information

SHQ1 assists in the assembly of H/ACA-box ribonucleoproteins that function in the processing of ribosomal RNAs modification of spliceosomal small nuclear RNAs and stabilization of telomerase (see MIM 602322) (Grozdanov et al. 2009 [PubMed 19383767]).[supplied by OMIM Dec 2010]

Gene Symbol:   SHQ1
Gene Name:   SHQ1 homolog (S. cerevisiae)
Aliases:   DKFZp686H07226, FLJ10539, Shq1p
RefSeq:   NC_000003.11 NT_022459.15
Ensembl:   ENSG00000144736
Entrez:   55164
Chromosome Mapping:   3p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.320000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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