PrimePCR™ Probe Assay: GTSE1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024969
Assay Design:   Exonic
Chromosome Location:   22:46704273-46704434question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000454366 ENST00000361934

Gene Information

The protein encoded by this gene is only expressed in the S and G2 phases of the cell cycle where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53 shuttling it out of the nucleus and repressing its ability to induce apoptosis. [provided by RefSeq Jul 2008]

Gene Symbol:   GTSE1
Gene Name:   G-2 and S-phase expressed 1
Aliases:   B99
RefSeq:   NC_000022.10 NG_012173.1 NT_011520.12
Ensembl:   ENSG00000075218
Entrez:   51512
UniGene:   Hs.386189
Chromosome Mapping:   22q13.2-q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.060000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download