This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: GTSE1, Human
PrimePCR™ Template for Probe Assay: GTSE1, Human
The protein encoded by this gene is only expressed in the S and G2 phases of the cell cycle where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53 shuttling it out of the nucleus and repressing its ability to induce apoptosis. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.